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inverted fluorescence microscope  (Nikon)


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    Structured Review

    Nikon inverted fluorescence microscope
    Inverted Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 11616 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ti2+fluorescence+microscope/ECLIPSE+Ti2/pmc12934279-126-14-17
    Average 99 stars, based on 11616 article reviews
    inverted fluorescence microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Live Cell Imaging:

    Article Title: In‑Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and Testing
    Article Snippet: Cells were washed with phosphate-buffered saline 24 h post-transfection (PBS, Gibco cat. #10010-023) and stained with Hoechst 33342 nuclear dye (ThermoFisher, cat. #H3570) at a concentration of 0.5 μg/mL for 30 min. .. Following an additional PBS wash, live-cell imaging solution (LCIS, Invitrogen cat. #A59688DJ) was added immediately prior to imaging on a Nikon ECLIPSE Ti2 fluorescence microscope. .. HEK293 cells were seeded into a 12-well plate (CytoOne, cat. #CC7682-7512) at a concentration of 200,000 cells/well and incubated overnight to allow adherence.

    Imaging:

    Article Title: In‑Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and Testing
    Article Snippet: Cells were washed with phosphate-buffered saline 24 h post-transfection (PBS, Gibco cat. #10010-023) and stained with Hoechst 33342 nuclear dye (ThermoFisher, cat. #H3570) at a concentration of 0.5 μg/mL for 30 min. .. Following an additional PBS wash, live-cell imaging solution (LCIS, Invitrogen cat. #A59688DJ) was added immediately prior to imaging on a Nikon ECLIPSE Ti2 fluorescence microscope. .. HEK293 cells were seeded into a 12-well plate (CytoOne, cat. #CC7682-7512) at a concentration of 200,000 cells/well and incubated overnight to allow adherence.

    Article Title: Novel Small-Molecule Analogues of IU1 Ameliorate Amyloid-β Mediated Toxicity in Alzheimer's Disease Cell and Worm Models.
    Article Snippet: .. Imaging was performed using a Nikon Eclipse Ti2 fluorescence microscope (Nikon corporation, Tokyo, Japan). ..

    Article Title: Anti–PD-L1 therapy to prevent systemic immune suppression after polytraumatic brain injury in rats
    Article Snippet: Following three additional PBS washes, slides were mounted using ProLong Gold Antifade Mountant containing DAPI (Molecular Probes). .. Imaging was performed using a Nikon Eclipse Ti2 fluorescence microscope, and quantification was carried out with QuPath v0.4.3 open-source software. ..

    Fluorescence:

    Article Title: In‑Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and Testing
    Article Snippet: Cells were washed with phosphate-buffered saline 24 h post-transfection (PBS, Gibco cat. #10010-023) and stained with Hoechst 33342 nuclear dye (ThermoFisher, cat. #H3570) at a concentration of 0.5 μg/mL for 30 min. .. Following an additional PBS wash, live-cell imaging solution (LCIS, Invitrogen cat. #A59688DJ) was added immediately prior to imaging on a Nikon ECLIPSE Ti2 fluorescence microscope. .. HEK293 cells were seeded into a 12-well plate (CytoOne, cat. #CC7682-7512) at a concentration of 200,000 cells/well and incubated overnight to allow adherence.

    Article Title: Novel Small-Molecule Analogues of IU1 Ameliorate Amyloid-β Mediated Toxicity in Alzheimer's Disease Cell and Worm Models.
    Article Snippet: .. Imaging was performed using a Nikon Eclipse Ti2 fluorescence microscope (Nikon corporation, Tokyo, Japan). ..

    Article Title: Anti–PD-L1 therapy to prevent systemic immune suppression after polytraumatic brain injury in rats
    Article Snippet: Following three additional PBS washes, slides were mounted using ProLong Gold Antifade Mountant containing DAPI (Molecular Probes). .. Imaging was performed using a Nikon Eclipse Ti2 fluorescence microscope, and quantification was carried out with QuPath v0.4.3 open-source software. ..

    Article Title: Spatially concentrated adenine base editors efficiently correct PLP1 mutations in oligodendrocytes
    Article Snippet: .. Stained sections were captured using a Nikon ECLIPSE Ti2 fluorescence microscope and analyzed using ImageJ software. ..

    Article Title: The BRCA1-A complex restricts replication fork reversal-dependent DNA repair in ATM deficient cells
    Article Snippet: Slides were stained with DAPI (D9542, Sigma Aldrich, 4 μg/ml diluted in 1X PBS) and mounted with ProLong Gold Antifade mountant (P36930, Thermo Scientific). .. Images were acquired by Nikon Eclipse Ti2 fluorescence microscope using a 100× Oil objective lens or a 60× Oil DIC N2 objective lens of Nikon Eclipse 80i fluorescence microscope. .. SIRF-PLA foci were quantified using CellProfiler 4.2.5 software and normalized with the average EdU-EdU signal in each sample.

    Article Title: The BRCA1-A complex restricts replication fork reversal-dependent DNA repair in ATM deficient cells
    Article Snippet: The slides were air dried, recovered overnight and stained with Giemsa (48900, Sigma-Aldrich) followed by mounting using Prolong Gold antifade mountant (P36930, ThermoFisher). .. Images were acquired using Nikon Eclipse Ti2 fluorescence microscope equipped with a 100× Oil objective lens. .. Cells were lysed in 2X LDS sample buffer (NP0007, Thermo Scientific) supplemented with benzonase (E1014, Sigma-Aldrich) and 1.5 mM MgCl2.

    Article Title: Spatially concentrated adenine base editors efficiently correct PLP1 mutations in oligodendrocytes.
    Article Snippet: .. Stained sections were capured using a Nikon ECLIPSE Ti2 fluorescence microscope and analyzed using ImageJ software. ..

    Article Title: Spatially concentrated adenine base editors efficiently correct PLP1 mutations in oligodendrocytes
    Article Snippet: For secondary immunostaining, Alexa Fluor antibodies (Thermo Fisher) were used at 1 μg/ml, and 4′,6-diamidino-2-phenylindole (DAPI, Sigma, D8417, 100 ng/ml) was used to identify nuclei. .. Images were captured using Nikon ECLIPSE Ti2 fluorescence microscope and analyzed using ImageJ software. ..

    Microscopy:

    Article Title: In‑Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and Testing
    Article Snippet: Cells were washed with phosphate-buffered saline 24 h post-transfection (PBS, Gibco cat. #10010-023) and stained with Hoechst 33342 nuclear dye (ThermoFisher, cat. #H3570) at a concentration of 0.5 μg/mL for 30 min. .. Following an additional PBS wash, live-cell imaging solution (LCIS, Invitrogen cat. #A59688DJ) was added immediately prior to imaging on a Nikon ECLIPSE Ti2 fluorescence microscope. .. HEK293 cells were seeded into a 12-well plate (CytoOne, cat. #CC7682-7512) at a concentration of 200,000 cells/well and incubated overnight to allow adherence.

    Article Title: Novel Small-Molecule Analogues of IU1 Ameliorate Amyloid-β Mediated Toxicity in Alzheimer's Disease Cell and Worm Models.
    Article Snippet: .. Imaging was performed using a Nikon Eclipse Ti2 fluorescence microscope (Nikon corporation, Tokyo, Japan). ..

    Article Title: Anti–PD-L1 therapy to prevent systemic immune suppression after polytraumatic brain injury in rats
    Article Snippet: Following three additional PBS washes, slides were mounted using ProLong Gold Antifade Mountant containing DAPI (Molecular Probes). .. Imaging was performed using a Nikon Eclipse Ti2 fluorescence microscope, and quantification was carried out with QuPath v0.4.3 open-source software. ..

    Article Title: Spatially concentrated adenine base editors efficiently correct PLP1 mutations in oligodendrocytes
    Article Snippet: .. Stained sections were captured using a Nikon ECLIPSE Ti2 fluorescence microscope and analyzed using ImageJ software. ..

    Article Title: The BRCA1-A complex restricts replication fork reversal-dependent DNA repair in ATM deficient cells
    Article Snippet: Slides were stained with DAPI (D9542, Sigma Aldrich, 4 μg/ml diluted in 1X PBS) and mounted with ProLong Gold Antifade mountant (P36930, Thermo Scientific). .. Images were acquired by Nikon Eclipse Ti2 fluorescence microscope using a 100× Oil objective lens or a 60× Oil DIC N2 objective lens of Nikon Eclipse 80i fluorescence microscope. .. SIRF-PLA foci were quantified using CellProfiler 4.2.5 software and normalized with the average EdU-EdU signal in each sample.

    Article Title: The BRCA1-A complex restricts replication fork reversal-dependent DNA repair in ATM deficient cells
    Article Snippet: The slides were air dried, recovered overnight and stained with Giemsa (48900, Sigma-Aldrich) followed by mounting using Prolong Gold antifade mountant (P36930, ThermoFisher). .. Images were acquired using Nikon Eclipse Ti2 fluorescence microscope equipped with a 100× Oil objective lens. .. Cells were lysed in 2X LDS sample buffer (NP0007, Thermo Scientific) supplemented with benzonase (E1014, Sigma-Aldrich) and 1.5 mM MgCl2.

    Article Title: Spatially concentrated adenine base editors efficiently correct PLP1 mutations in oligodendrocytes.
    Article Snippet: .. Stained sections were capured using a Nikon ECLIPSE Ti2 fluorescence microscope and analyzed using ImageJ software. ..

    Article Title: Spatially concentrated adenine base editors efficiently correct PLP1 mutations in oligodendrocytes
    Article Snippet: For secondary immunostaining, Alexa Fluor antibodies (Thermo Fisher) were used at 1 μg/ml, and 4′,6-diamidino-2-phenylindole (DAPI, Sigma, D8417, 100 ng/ml) was used to identify nuclei. .. Images were captured using Nikon ECLIPSE Ti2 fluorescence microscope and analyzed using ImageJ software. ..

    Software:

    Article Title: Anti–PD-L1 therapy to prevent systemic immune suppression after polytraumatic brain injury in rats
    Article Snippet: Following three additional PBS washes, slides were mounted using ProLong Gold Antifade Mountant containing DAPI (Molecular Probes). .. Imaging was performed using a Nikon Eclipse Ti2 fluorescence microscope, and quantification was carried out with QuPath v0.4.3 open-source software. ..

    Staining:

    Article Title: Spatially concentrated adenine base editors efficiently correct PLP1 mutations in oligodendrocytes
    Article Snippet: .. Stained sections were captured using a Nikon ECLIPSE Ti2 fluorescence microscope and analyzed using ImageJ software. ..

    Article Title: Spatially concentrated adenine base editors efficiently correct PLP1 mutations in oligodendrocytes.
    Article Snippet: .. Stained sections were capured using a Nikon ECLIPSE Ti2 fluorescence microscope and analyzed using ImageJ software. ..



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    Nikon nikon inverted fluorescence microscope
    Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron <t>microscope</t> images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead <t>fluorescence</t> images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    Nikon fluorescence microscope
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    Image Search Results


    Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron microscope images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead fluorescence images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions

    doi: 10.1016/j.mtbio.2026.103015

    Figure Lengend Snippet: Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron microscope images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead fluorescence images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Following the incubation, fluorescence images were captured using a Nikon inverted fluorescence microscope (Nikon, Japan, Modle: Eclipse Ti2-E).

    Techniques: Fourier Transform Infrared Spectroscopy, Spectroscopy, Imaging, Microscopy, Pore Size, Fluorescence, Co-Culture Assay, Cell Culture